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Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files BJM
Silva,Raquel Assed Bezerra da; Leonardo,Mário Roberto; Faccioli,Lúcia Helena; Medeiros,Alexandra Ivo; Nelson-Filho,Paulo.
This study evaluated the presence of lipopolysaccharide (LPS) in endodontic files after in vitro contamination with E. coli LPS and sterilization in dry heat or wet heat, with or without previous immersion in a Ca(OH)2 suspension. LPS was quantified using the Kinetic QCL TM test and data were analyzed statistically by the t-test. LPS was quantified only in the contaminated group, not submitted to any immersion or sterilization procedure (0.262±0.1296 endotoxin units/mL) (p=0.0003). In conclusion, both wet heat and dry heat sterilization were effective in inactivating LPS without the need of previous immersion of the files in a Ca(OH)2 suspension.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endotoxin; LPS; Endodontics; Dry heat; Wet heat.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200015
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SEED DORMANCY BREAKING TREATMENTS FORAFRICAN PURSLANE (Zaleya pentandra) Planta Daninha
MUNAWAR,S.; NAEEM,M.; ALI,H.H.; JAMIL,M.; IQBAL,M.; NAZIR,M.Q.; BALAL,R.M.; SAFDAR,M.E..
ABSTRACT Understanding the mechanisms involved in releasing seed dormancy is crucial for effective plant management and renewal of species in the arid zone. Zaleya pentandra is an emerging invasive weed of the arid areas of Pakistan. We investigated the effects of different dormancy breaking treatments on the germination of Z. pentandra seeds. Seeds were treated with hot water (by placing them in boiling water for 5, 15, 30, 60, 90, 120, and 150 min), dry heat (by placing them in a preheated oven at 70 oC for 1, 2, and 4 hours; at 70 oC for 1, 2, 3, and 4 days, and at 200 oC for 5, 10, 15, 30, and 45 min) and stratification (by placing them at 2-5 ºC in a refrigerator for 5, 10, 30, and 60 min; for 3, 6, and 12 hours, and for 1, 2, 4, 8, 15, and 30 days)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Germination; Scarification; Stratification; Dry heat; Thiourea; Arid zone.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000400623
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